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基本信息(Basic Information) 仪器名称(Apparatus) 高效液相色谱-三重四级杆质谱联用仪
(HPLC-QQQMS)当前状态(Status) 离线状态(Off-Line) 厂商&型号(Company & Model) Agilent & HPLC 1260 - QQQ 6470 仪器负责人(Person in Charge) 张薇 电子邮件(Email) linda_z@sjtu.edu.cn 开放限制(Limited to) 对外开放(No Limitaion, Accessible to ALL) 使用类型(Operated by) 自主操作(User) 收费类型(Charging by) 计时计件(Time & Events) 购置时间(Acquisition Time) 2014-08-10 设备放置地点(Location) 3-108室 办公室(Office) 生物药学楼3-111室 联系电话(TEL.) 34206834 仪器介绍(Apparatus Introduction) 主要性能指标
1. 高效液相色谱(HPLC)
● 检测器：二极管阵列检测器(DAD)
● 液流模块：四元泵，0.001 ~ 5 mL/min
● 流量精度：≤0.07 % RSD 或 0.02 min SD
● 耐压：400 bar
● 数据采集速率：80 Hz
● 柱温箱：最高温度 80 ℃
● pH范围：1.0 ~ 12.5
2. 三重四级杆质谱(QQQ MS)
流速范围：0.1~5.0 ml/min
ESI 正离子灵敏度 S/N>75,000:1
ESI 负离子灵敏度 S/N>30,000:1
动态范围： 5 个数量级
仪器主要用途
环境分析
食品安全分析
生物分子分析
代谢物鉴定
药物研发
制药和生物制药行业的质量保证/质量控制注意事项
1. 样品极性较强，分子量范围 m/z 25-20,000，能溶于水、乙腈、甲醇、乙醇
2. 固体或冻干粉样品，含主要成分0-1 mg（须注明使用的溶剂：水、乙腈、甲醇、乙醇）
3. 溶液样品30-1000 μL，浓度10-1000 μg/mL
4. 必须去除样品中的磷酸盐、硫酸盐、盐酸盐等不挥发性盐类
5. 必须去除样品中的表面活性剂、强酸、强碱
6. 必须去除样品中的乙酸乙酯、氯仿、二氯甲烷、苯、环己烷、DMSO等有机试剂
7. 必须去除样品中的蛋白质、多糖、脂类物质
8. 血样、尿样、细胞组织样、微生物培养液等样品必须去除蛋白质、脂类和多糖，用流动相溶解，离心或膜过滤
9. 流动相中不可用三乙胺等离子对试剂
Specification
1. High Performance Liquid Chromatography (HPLC)
Detector: Diode Array Detector (DAD)
Quaternary pump: 0.001-10mL/min, 0.001 ml/min incremental
Flow precision: ≤ 0.07 % RSD or ≤ 0.02 min SD
Max pressure: 400Bar
Detector scan rate: 80Hz
Column Oven: Operating temperature up to 80℃
PH range：1.0-12.5
2. Triple Quadrupole Mass (QQQ MS)
Sensitivity(ESI Positive): S/N≥75,000:1
Sensitivity(ESI Negative): S/N≥30,000:1
Dynamic range: Up to 5 decadesApplication
Cautions
Environment analysis Food Safety analysis
Biomolecule analysis Metabolite analysis
QA/QC in pharmacy and bio-pharmacy
1. Sample would be polarity, mass range from 25 to 20,000, be solved in water, methanol, acetonitrile, ethanol.
2. For solid or powder sample, the concentration of the target would be 0-1 mg (the solvent should be water, acetonitrile, methanol, and ethanol.)
3. Volume of the saple is 30-1000 μl, the concentration is 10-1000 μg/ml.
4. Must remove the phosphate, sulfate, hydrochloric acid and other non-volatile salts from the sample.
5. Must remove the surfactant, strong acid and strong base.
6. Better remove ethyl acetate, chloroform, dichloromethane, benzene, cyclohexane, DMSO and other organic reagent.
7. Must remove protein, polysaccharide, and lipids.
8. These samples, such as blood, urine, tissue, cell, microbial culture medium, must be removed protein, polysaccharide, and lipids, solved with the mobile phase, and centrifuged or filtered.
9. Forbidden the usage of ion pair reagent, such as triethylamine.